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anti phospho mek 1 2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti phospho mek 1 2
    Anti Phospho Mek 1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mek+1+2/MEK1%2F2+Rabbit+mAb/bio_rxiv__64898__2026__03__05__709991-264-50-54
    Average 96 stars, based on 390 article reviews
    anti phospho mek 1 2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: A novel TRKB-activating internal tandem duplication characterizes a new mechanism of receptor tyrosine kinase activation
    Article Snippet: MEK 1/2 , Cell Signaling Technology , 9122 , AB_823567.

    Membrane:

    Article Title: Exploiting Paradoxical Activation of Oncogenic MAPK Signaling by Targeting Mitochondria to Sensitize NRAS Mutant-Melanoma to Vemurafenib
    Article Snippet: Briefly, protein mixture in cell lysates was resolved using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA). .. After membrane blocking with 2.5% BSA in TRIS-buffered saline containing Tween 20, proteins were subsequently detected using the following primary antibodies: β-actin (#3700), COX IV (#4844), MFN1 (#14739), MFN2 (#9482), OPA1 (#80471), DRP1 (#8570), RAS (#3965), BRAF (#14814), pBRAF(#2696), MEK 1/2 (#9126), pMEK 1/2 (#9154), p44/42 MAPK (ERK 1/2) (#4695), and p-p44/42 MAK (ERK 1/2) (#4695) (Cell Signaling, USA). .. After subsequent incubation with the respective HRP-conjugated secondary antibodies, anti-mouse (#7076) or anti-rabbit (#7074) (Cell Signaling, USA), the labeled proteins were revealed with chemiluminescent detection kit PierceTM ECL Plus Substrate (Thermo Fisher Scientific, USA) and digitized using the ChemiDocTM MP Imaging software v5.0 (Bio-Rad, USA).

    Article Title: Exploiting Paradoxical Activation of Oncogenic MAPK Signaling by Targeting Mitochondria to Sensitize NRAS Mutant-Melanoma to Vemurafenib.
    Article Snippet: Briefly, protein mixture in cell lysates was resolved using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA). .. After membrane blocking with 2.5% BSA in TRIS-buffered saline containing Tween 20, proteins were subsequently detected using the following primary antibodies: β-actin (#3700), COX IV (#4844), MFN1 (#14739), MFN2 (#9482), OPA1 (#80471), DRP1 (#8570), RAS (#3965), BRAF (#14814), pBRAF(#2696), MEK 1/2 (#9126), pMEK 1/2 (#9154), p44/42 MAPK (ERK 1/2) (#4695), and p-p44/42 MAK (ERK 1/2) (#4695) (Cell Signaling, USA). .. After subsequent incubation with the respective HRP-conjugated secondary antibodies, anti-mouse (#7076) or anti-rabbit (#7074) (Cell Signaling, USA), the labeled proteins were revealed with chemiluminescent detection kit PierceTM ECL Plus Substrate (Thermo Fisher Scientific, USA) and digitized using the ChemiDocTM MP Imaging software v5.0 (Bio-Rad, USA).

    Blocking Assay:

    Article Title: Exploiting Paradoxical Activation of Oncogenic MAPK Signaling by Targeting Mitochondria to Sensitize NRAS Mutant-Melanoma to Vemurafenib
    Article Snippet: Briefly, protein mixture in cell lysates was resolved using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA). .. After membrane blocking with 2.5% BSA in TRIS-buffered saline containing Tween 20, proteins were subsequently detected using the following primary antibodies: β-actin (#3700), COX IV (#4844), MFN1 (#14739), MFN2 (#9482), OPA1 (#80471), DRP1 (#8570), RAS (#3965), BRAF (#14814), pBRAF(#2696), MEK 1/2 (#9126), pMEK 1/2 (#9154), p44/42 MAPK (ERK 1/2) (#4695), and p-p44/42 MAK (ERK 1/2) (#4695) (Cell Signaling, USA). .. After subsequent incubation with the respective HRP-conjugated secondary antibodies, anti-mouse (#7076) or anti-rabbit (#7074) (Cell Signaling, USA), the labeled proteins were revealed with chemiluminescent detection kit PierceTM ECL Plus Substrate (Thermo Fisher Scientific, USA) and digitized using the ChemiDocTM MP Imaging software v5.0 (Bio-Rad, USA).

    Article Title: Exploiting Paradoxical Activation of Oncogenic MAPK Signaling by Targeting Mitochondria to Sensitize NRAS Mutant-Melanoma to Vemurafenib.
    Article Snippet: Briefly, protein mixture in cell lysates was resolved using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA). .. After membrane blocking with 2.5% BSA in TRIS-buffered saline containing Tween 20, proteins were subsequently detected using the following primary antibodies: β-actin (#3700), COX IV (#4844), MFN1 (#14739), MFN2 (#9482), OPA1 (#80471), DRP1 (#8570), RAS (#3965), BRAF (#14814), pBRAF(#2696), MEK 1/2 (#9126), pMEK 1/2 (#9154), p44/42 MAPK (ERK 1/2) (#4695), and p-p44/42 MAK (ERK 1/2) (#4695) (Cell Signaling, USA). .. After subsequent incubation with the respective HRP-conjugated secondary antibodies, anti-mouse (#7076) or anti-rabbit (#7074) (Cell Signaling, USA), the labeled proteins were revealed with chemiluminescent detection kit PierceTM ECL Plus Substrate (Thermo Fisher Scientific, USA) and digitized using the ChemiDocTM MP Imaging software v5.0 (Bio-Rad, USA).

    Saline:

    Article Title: Exploiting Paradoxical Activation of Oncogenic MAPK Signaling by Targeting Mitochondria to Sensitize NRAS Mutant-Melanoma to Vemurafenib
    Article Snippet: Briefly, protein mixture in cell lysates was resolved using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA). .. After membrane blocking with 2.5% BSA in TRIS-buffered saline containing Tween 20, proteins were subsequently detected using the following primary antibodies: β-actin (#3700), COX IV (#4844), MFN1 (#14739), MFN2 (#9482), OPA1 (#80471), DRP1 (#8570), RAS (#3965), BRAF (#14814), pBRAF(#2696), MEK 1/2 (#9126), pMEK 1/2 (#9154), p44/42 MAPK (ERK 1/2) (#4695), and p-p44/42 MAK (ERK 1/2) (#4695) (Cell Signaling, USA). .. After subsequent incubation with the respective HRP-conjugated secondary antibodies, anti-mouse (#7076) or anti-rabbit (#7074) (Cell Signaling, USA), the labeled proteins were revealed with chemiluminescent detection kit PierceTM ECL Plus Substrate (Thermo Fisher Scientific, USA) and digitized using the ChemiDocTM MP Imaging software v5.0 (Bio-Rad, USA).

    Article Title: Causal relevance of the JAK/STAT pathway for cardioprotection via cold acclimation.
    Article Snippet: Using a Wet Blot Module (Bio-Rad), proteins were electro-transferred onto nitrocellulose membranes (0.2–0.45 μm pore size; Bio-Rad) at 100 V for 90 min 10,11,26. .. Membranes were then blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.05% Tween 20 (20 mM Tris, 500 mM NaCl) for 1 h and incubated overnight at 4 °C with polyclonal antibodies against STAT3 (CS30835; Cell Signaling Technology), pSTAT3-Y705 (CS4113), pSTAT3-S727 (Ab32143; Abcam), Bcl-2 (SAB4500003), BAX (Ab7977), Caspase-3 (Ab13847), p38 (sc-SC535; Santa Cruz), p-p38 (sc-17852), p-p38-T180/Y182 (sc-17852-R), Akt (SC8312), p-Akt-S473 (CS9271); MEK-1/2 (cs-9122, Cell Signaling Technology), pMEK-1/2-S217/221 (cs-9121, Cell Signaling Technology), ERK-1/2 (cs-9102, Cell Signaling Technology), pERK-1/2 (cs-9101, Cell Signaling Technology. .. The following day, membranes were washed and incubated with HRP-conjugated secondary antibodies—anti-rabbit (Sigma-Aldrich, A9169) or anti-mouse (Invitrogen, 31432).

    Article Title: Exploiting Paradoxical Activation of Oncogenic MAPK Signaling by Targeting Mitochondria to Sensitize NRAS Mutant-Melanoma to Vemurafenib.
    Article Snippet: Briefly, protein mixture in cell lysates was resolved using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules CA, USA). .. After membrane blocking with 2.5% BSA in TRIS-buffered saline containing Tween 20, proteins were subsequently detected using the following primary antibodies: β-actin (#3700), COX IV (#4844), MFN1 (#14739), MFN2 (#9482), OPA1 (#80471), DRP1 (#8570), RAS (#3965), BRAF (#14814), pBRAF(#2696), MEK 1/2 (#9126), pMEK 1/2 (#9154), p44/42 MAPK (ERK 1/2) (#4695), and p-p44/42 MAK (ERK 1/2) (#4695) (Cell Signaling, USA). .. After subsequent incubation with the respective HRP-conjugated secondary antibodies, anti-mouse (#7076) or anti-rabbit (#7074) (Cell Signaling, USA), the labeled proteins were revealed with chemiluminescent detection kit PierceTM ECL Plus Substrate (Thermo Fisher Scientific, USA) and digitized using the ChemiDocTM MP Imaging software v5.0 (Bio-Rad, USA).

    Incubation:

    Article Title: Causal relevance of the JAK/STAT pathway for cardioprotection via cold acclimation.
    Article Snippet: Using a Wet Blot Module (Bio-Rad), proteins were electro-transferred onto nitrocellulose membranes (0.2–0.45 μm pore size; Bio-Rad) at 100 V for 90 min 10,11,26. .. Membranes were then blocked with 5% non-fat dry milk in Tris-buffered saline containing 0.05% Tween 20 (20 mM Tris, 500 mM NaCl) for 1 h and incubated overnight at 4 °C with polyclonal antibodies against STAT3 (CS30835; Cell Signaling Technology), pSTAT3-Y705 (CS4113), pSTAT3-S727 (Ab32143; Abcam), Bcl-2 (SAB4500003), BAX (Ab7977), Caspase-3 (Ab13847), p38 (sc-SC535; Santa Cruz), p-p38 (sc-17852), p-p38-T180/Y182 (sc-17852-R), Akt (SC8312), p-Akt-S473 (CS9271); MEK-1/2 (cs-9122, Cell Signaling Technology), pMEK-1/2-S217/221 (cs-9121, Cell Signaling Technology), ERK-1/2 (cs-9102, Cell Signaling Technology), pERK-1/2 (cs-9101, Cell Signaling Technology. .. The following day, membranes were washed and incubated with HRP-conjugated secondary antibodies—anti-rabbit (Sigma-Aldrich, A9169) or anti-mouse (Invitrogen, 31432).



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